| 1. | Study on the pathogenicity of heterokaryon and parental strains of paecilomyces cicadae for aphids
蝉拟青霉异核体菌株和亲本菌株对蚜虫致病力的研究 |
| 2. | The total amount of avermectins b were only about 0 . 5 ~ 2 % of those produced by the parental strain olm73 - 12 . this study suggests that t
在281个突变株中，还筛选到了2个突变株，其发酵产物几乎全部为有效组分阿维菌素b1 ， b2组分仅占5 。 |
| 3. | Through the test , the strain of y7 , which had the highest content of trehalose and strong tolerance to sodium chloride and acetic acid , was defined as a parental strain
综合耐性实验和海藻糖积累实验的结果，确定了海藻糖含量高，渗透压和乙酸耐性最强的y7菌株为本研究出发菌株。 |
| 4. | The resulting derivative strains produced antifungal activity in a manner different from the parental strain , indicating the original promoter was replaced with the engineered promoters and the epitopes
衍生菌株合成抗真菌抗生素的产量与亲本菌株不同，表明fr - 008基因的原始启动子已被改造后的启动子和抗原决定簇所代替。 |
| 5. | Comparing with the y7 , the specific growth rate of mutant increased by 22 . 7 % , the activities of neutral and acid trehalase droped by 19 . 2 % and 6 . 7 % , respectively . under the non - optimized condition , the production of trehalose increased by 57 . 2 % compared with that of parental strain y7
与原菌株y7相比较，对数期比生长速率提高22 . 7 ，酸性海藻糖分解酶活性下降19 . 2 ，中性海藻糖分解酶活性下降26 . 7 ，胞内海藻糖含量提高了57 . 2 。 |
| 6. | Shaking flask experiments and hplc analyses showed that 99 isolates still produced four components of avermectins b , in which the yields of avermectins b in 82 isolates were only about 0 . 1 ~ 2 % of those produced by the parental strain olm73 - 12 . 2 of 82 isolates were confirmed to be the correct gene replacement mutants by pcr and sequence analysis . the mutant only producing avermectins bl was not detected
摇瓶发酵和hplc分析结果表明，有99个突变株仍然产阿维菌素b的4个组分，其中82株的发酵单位很低，仅为出发菌株olm73 - 12的0 . 1 2 ，从中挑取2株经pcr扩增和测序验证，均发生了正确的基因取代；没有检测到仅产b1的突变株，这表明阿维菌素b2组分的产生并不是因为阿维菌素pks上dh2的部分活性所造成。 |
| 7. | Plant assay was performed with the wild - type strain gx201 , the mutant strain gx217 and the function recovered strain rgx217 . the results indicate that the mutant strain gx217 showed a day delay in nodulation time and its abilities of nodule formation efficiency and competitive nodulation were apparently decreased compared with the parental strain gx201 respectively
通过对野生型菌株gx201 ，突变体菌株gx217及其功能互补菌株rgx217进行植株试验，发现突变体菌株gx217在结瘤时间方面较野生型菌株gx201慢一天，其结瘤效率及竞争结瘤能力均比gx201明显降低。 |
| 8. | The donkey - adapted strain of eiav ( dv116 ) , parental strain of eiav - dla , is a highly virulent isolate which was developed by sequential passaging a virulent eiav strain in donkeys in 1970s . the donkeys inoculated with fatal doses of eiav d strain can always be killed within in the first acute disease period . in this study , we constructed a full - length provirus dna clone of dv116 by pcr
本实验中将eiav驴强毒dv体外感染驴白细胞培养物，一定时间后收获病毒(本文中简称dv116 ) ，提取eiav前病毒dna ，以pcr法分别扩增并克隆了包含全长基因的三段前病毒dna片段，以双脱氧法测定了dv116病毒全基因序列共8236个核苷酸。 |
| 9. | There is no acrystalliferous derivative was screened after treating 4 . 5 g / ml ethidium bromide , but after elevating growth temperature of ybt - 1463 and other 8 bacillus thuringiensis parental strains to 42 , 9 acrystalliferous derivative were obtained . a series of partially plasmid - cured derivatives were further obtained from 3 acrystalliferous derivatives originated from wild - type strain ybt - 1463 by elevating temperature to 44
用限量培养基和42培养9株野生菌株，结果筛选到9株cry ~ -突变株；继续升温至44来培养其中的三个cry ~ -突变株，得到内生质粒被进一步消除的突变株。 |
